منابع مشابه
Recombineering
Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phage-derived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in E. coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of ...
متن کاملCRMAGE: CRISPR Optimized MAGE Recombineering
A bottleneck in metabolic engineering and systems biology approaches is the lack of efficient genome engineering technologies. Here, we combine CRISPR/Cas9 and λ Red recombineering based MAGE technology (CRMAGE) to create a highly efficient and fast method for genome engineering of Escherichia coli. Using CRMAGE, the recombineering efficiency was between 96.5% and 99.7% for gene recoding of thr...
متن کاملCRISPR–Cas9-assisted recombineering in Lactobacillus reuteri
Clustered regularly interspaced palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) nuclease protect bacteria and archeae from foreign DNA by site-specific cleavage of incoming DNA. Type-II CRISPR-Cas systems, such as the Streptococcus pyogenes CRISPR-Cas9 system, can be adapted such that Cas9 can be guided to a user-defined site in the chromosome to introduce double-stranded breaks. ...
متن کاملCreating Porcine Biomedical Models Through Recombineering
Recent advances in genomics provide genetic information from humans and other mammals (mouse, rat, dog and primates) traditionally used as models as well as new candidates (pigs and cattle). In addition, linked enabling technologies, such as transgenesis and animal cloning, provide innovative ways to design and perform experiments to dissect complex biological systems. Exploitation of genomic i...
متن کاملManipulating the Mouse Genome Using Recombineering
Animal models are routinely used to understand the etiology of human genetic disorders, as well as to develop preclinical models to test the efficacy of novel therapies. Although there are speciesspecific variations based on gene function studies, the mouse is the most reliable and commonly used model system, mainly because of its genetic and physiological similarity to humans. In addition, adv...
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ژورنال
عنوان ژورنال: Bacteriophage
سال: 2012
ISSN: 2159-7081
DOI: 10.4161/bact.18778